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thp-1, thp-1 asc-gfp, thp-1 nlrp3 deficient cell lines  (InvivoGen)


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    InvivoGen thp-1, thp-1 asc-gfp, thp-1 nlrp3 deficient cell lines
    B. pertussis induces NLRP3 inflammasome activation in human MΦ-like <t>THP-1</t> cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.
    Thp 1, Thp 1 Asc Gfp, Thp 1 Nlrp3 Deficient Cell Lines, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thp+1+asc+gfp+cell+line/RAW-Blue+cells/pmc06718514-53-1-10
    Average 96 stars, based on 432 article reviews
    thp-1, thp-1 asc-gfp, thp-1 nlrp3 deficient cell lines - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages"

    Article Title: Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2019.02030

    B. pertussis induces NLRP3 inflammasome activation in human MΦ-like THP-1 cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.
    Figure Legend Snippet: B. pertussis induces NLRP3 inflammasome activation in human MΦ-like THP-1 cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.

    Techniques Used: Activation Assay, Cytotoxicity Assay, Positive Control, Incubation

    Related Articles

    Activation Assay:

    Article Title: Overcoming Preclinical Safety Obstacles to Discover ( S )- N -((1,2,3,5,6,7-Hexahydro- s -indacen-4-yl)carbamoyl)-6-(methylamino)-6,7-dihydro-5 H -pyrazolo[5,1- b ][1,3]oxazine-3-sulfonamide (GDC-2394): A Potent and Selective NLRP3 Inhibitor.
    Article Snippet: Inappropriate activation of the NLRP3 inflammasome has been implicated in multiple inflammatory and autoimmune diseases.. Herein, we aimed to develop novel NLRP3 inhibitors that could minimize the risk of drug-induced liver injury.. Lipophilic ligand efficiency was used as a guiding metric to identify a s e r i e s o f 6 , 7 d i hyd ro -5H-py r a zo l o [5 , 1 -b ] [1 , 3 ] oxazinesulfonylureas.

    Article Title: Sulfonimidamide compounds as inhibitors of interleukin-1 activity
    Article Snippet: .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for inflammasome activation assay. ..

    Article Title: Sulfonylurea compounds as inhibitors of interleukin-1 activity
    Article Snippet: .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for infammasome activation assay. .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for infammasome activation assay.

    Cell Culture:

    Article Title: Sulfonimidamide compounds as inhibitors of interleukin-1 activity
    Article Snippet: .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for inflammasome activation assay. ..

    Article Title: Sulfonylurea compounds as inhibitors of interleukin-1 activity
    Article Snippet: .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for infammasome activation assay. .. Cell Culture: THP-1 ASC-GFP cell line was purchased from Invivogen, San Diego, for infammasome activation assay.



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    B. pertussis induces NLRP3 inflammasome activation in human MΦ-like <t>THP-1</t> cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.
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    B. pertussis induces NLRP3 inflammasome activation in human MΦ-like THP-1 cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.

    Journal: Frontiers in Immunology

    Article Title: Activation of Human NK Cells by Bordetella pertussis Requires Inflammasome Activation in Macrophages

    doi: 10.3389/fimmu.2019.02030

    Figure Lengend Snippet: B. pertussis induces NLRP3 inflammasome activation in human MΦ-like THP-1 cells. MΦ-like THP-1 cells were stimulated with B. pertussis (Tohama I, MOI = 100, 10 or 1) or left untreated for 22 h in the presence (dashed bars) or absence (clear bars) of the caspase inhibitor, Z-VAD-FMK. (A) IL-1β ( n = 5) and (C) IL-6 ( n = 3) were measured in the supernatant of at least three independent experiments. (B) LDH release ( n = 3) was determined with a Cytotoxicity Assay. LDH release is shown as a percentage of the LDH released relative to the percentage of LDH released in the positive control, LPS + nigericin (100% cell death), for NLRP3 activation. (D–F) MΦ-like NLRP3 deficient THP-1 cells (horizontal lines) were incubated with B. pertussis (Tohama I, MOI = 10) for 22 h. (D) IL-1β ( n = 3) and ( F ) IL-6 ( n = 3) levels were measured in the supernatant using ELISAs. (E) The LDH released by MΦ-like NLRP3 deficient THP-1 cells ( n = 4) was shown as relative to the LDH release from fully lysed cultures. Results are expressed as medians with interquartile range from at least three independent experiments. Black dots represent the average values from each experiments.

    Article Snippet: The THP-1, THP-1 ASC-GFP, and THP-1 NLRP3 deficient cell lines (InvivoGen) were cultured in RPMI culture medium at 37°C and 5% CO 2 .

    Techniques: Activation Assay, Cytotoxicity Assay, Positive Control, Incubation